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Characterization of a partial cDNA clone encoding porcine skeletal muscle titin: comparison with rabbit and mouse
J D Fritz1, J A Wolff, M L Greaser
1Department of Meat and Animal Science, Waisman Center, University of Wisconsin-Madison 53706.
Abstract:
1. A cDNA fragment encoding 571 amino acid residues of porcine skeletal muscle titin was isolated from total RNA using RNA-PCR. 2. The porcine titin clone hybridized to a large RNA species (> 23 kb) in rabbit cardiac muscle, rabbit skeletal muscle and porcine skeletal muscle. 3. The porcine skeletal muscle titin clone encoded two types of 100-residue motifs and its amino acid sequence was 96% and 93% identical to the corresponding sequence of rabbit and mouse skeletal muscle titin, respectively. These results suggest that the titin sequence is highly conserved among mammalian species.
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