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Related Experiment Videos

Structure of clathrin-coated vesicles from small-angle scattering experiments

J S Pedersen1

  • 1Department of Solid State Physics, Risø National Laboratory, Roskilde, Denmark.

European Biophysics Journal : EBJ
|January 1, 1993
PubMed
Summary

This study analyzed scattering data from coated vesicles, revealing a dense protein shell within the clathrin layer, suggesting proteins are integrated into the lipid membrane. Reassembled coats mimic native structures, with a denser central shell indicating membrane replacement by protein.

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Area of Science:

  • Biophysics
  • Structural Biology
  • Cell Biology

Background:

  • Coated vesicles are crucial for intracellular transport.
  • Understanding their structure, particularly the protein-lipid interactions, is key to deciphering their function.

Purpose of the Study:

  • To model the structural organization of coated vesicles using scattering data.
  • To determine the distribution and density of protein and lipid components within coated vesicles.

Main Methods:

  • Analysis of small-angle neutron and X-ray scattering data.
  • Application of a spherical symmetry model with polydispersity.
  • Contrast variation measurements using D2O solvent concentrations.

Main Results:

Related Experiment Videos

  • Coated vesicles feature a low-density outer clathrin shell and a high-density inner shell of accessory proteins and receptors.
  • The lipid membrane, identified via neutron contrast variation, overlaps with the inner protein shell, indicating protein incorporation.
  • Reassembled coats show structural similarity to native coats, but with a denser central shell.

    • Conclusions:

    • Accessory polypeptides are integrated into the lipid membrane of coated vesicles.
    • The high protein density in the central region suggests limited hydration.
    • Reassembled coats serve as a model, highlighting protein's role in membrane structure.