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Modified celluloses for erythrocyte deformability fractionation

C Acquaye1, R M Johnson

  • 1Department of Biochemistry, Wayne State University Medical School, Detroit, MI.

Experimental Hematology
|September 1, 1993
PubMed
Summary
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Modified cellulose columns improve the separation of red blood cells by deformability. These new methods enhance recovery rates for both normal and sickle cells, yielding more rigid cells.

Area of Science:

  • Hematology
  • Biomaterials Science
  • Cell Biology

Background:

  • Cellulose columns have limitations in separating erythrocytes by deformability, often resulting in variable and incomplete recoveries.
  • Improving erythrocyte separation is crucial for understanding cell mechanics and diagnosing related diseases.

Purpose of the Study:

  • To evaluate the efficacy of modified cellulose columns (propylaminocarbonylmethyl cellulose [PAC] and butylisourea cellulose [BIC]) for improved erythrocyte separation based on deformability.
  • To enhance the recovery rates of both normal and sickle cells during separation.
  • To characterize the deformability of cells eluted in later fractions.

Main Methods:

  • Utilized propylaminocarbonylmethyl cellulose (PAC) and butylisourea cellulose (BIC) modified cellulose columns for erythrocyte separation.

Related Experiment Videos

  • Applied both normal and sickle erythrocytes to the prepared columns.
  • Collected and analyzed eluted cell fractions to determine recovery rates and deformability.

    • Main Results:

    • Modified cellulose columns (PAC and BIC) significantly increased the recovery of applied erythrocytes compared to unmodified cellulose.
    • Achieved reasonable yields of rigid cells in the late eluting fractions.
    • Sickle cells demonstrated a 95% recovery rate, and late eluting cells exhibited a sharply reduced deformability index.

    Conclusions:

    • Propylaminocarbonylmethyl cellulose (PAC) and butylisourea cellulose (BIC) columns represent an effective advancement for separating erythrocytes by deformability.
    • These modified cellulose matrices offer improved recovery yields, particularly for sickle cells, and facilitate the isolation of less deformable cells.