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Two-phase partitioning applied to changes in phospholipid asymmetry

C Tilcock1, D Fisher

  • 1Department of Radiology, University of British Columbia, Vancouver, Canada.

Biochemical Society Transactions
|May 1, 1993
PubMed
Summary

Aqueous two-phase polymer partition is a simple, inexpensive method to determine lipid asymmetry in membranes. This technique effectively monitors changes in lipid distribution within model and biological systems.

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Area of Science:

  • Biochemistry
  • Membrane Biophysics
  • Analytical Chemistry

Background:

  • Lipid asymmetry is crucial for biological membrane function.
  • Existing methods for assessing lipid asymmetry can be complex or costly.

Purpose of the Study:

  • To evaluate aqueous two-phase polymer partition as a method for determining lipid asymmetry.
  • To demonstrate the technique's applicability in model and biological membrane systems.

Main Methods:

  • Utilized aqueous two-phase polymer partition.
  • Established membrane potential across lipid bilayers in model systems.
  • Monitored calcium ion (Ca2+)-induced asymmetry in human erythrocytes.

Main Results:

  • Aqueous two-phase polymer partition proved effective for assessing lipid asymmetry.

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  • The method successfully demonstrated lipid asymmetry in model membranes.
  • The technique was useful for monitoring Ca2+-induced asymmetry in erythrocytes.

    • Conclusions:

    • Aqueous two-phase polymer partition is a valuable addition to lipid asymmetry analysis.
    • The methodology is straightforward, requires simple experimental setups, and uses inexpensive materials.
    • This technique offers a practical approach for studying lipid distribution in various membrane systems.