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Related Experiment Videos

Agr-related sequences in Staphylococcus lugdunensis

F Vandenesch1, S J Projan, B Kreiswirth

  • 1Département de Recherche en Bactériologie Médicale, Lyon, France.

FEMS Microbiology Letters
|July 15, 1993
PubMed
Summary

Sequences similar to the Staphylococcus aureus accessory gene regulator (agr) were found in S. lugdunensis. This regulator may control hemolytic activity, though it doesn't directly encode the hemolysin.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • The accessory gene regulator (agr) system in *Staphylococcus aureus* controls virulence factors.
  • Understanding *agr* homologs in other staphylococcal species is crucial for comparative genomics and understanding virulence evolution.

Purpose of the Study:

  • To investigate the presence and characteristics of *agr*-like sequences in *Staphylococcus lugdunensis*.
  • To determine if these sequences are transcribed and if they correlate with hemolytic activity.

Main Methods:

  • Southern blot analysis of 13 *S. lugdunensis* strains.
  • Sequencing of the *S. lugdunensis* *agr*-like locus (*agr-sl*).
  • Northern blot analysis of cellular RNA from six *S. lugdunensis* strains.

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Main Results:

  • *agr*-like sequences were detected in *S. lugdunensis* strains.
  • A transcript homologous to *S. aureus* RNAIII was identified in three strains.
  • These three strains produced hemolysin activity similar to *S. aureus* delta-hemolysin.
  • The *agr-sl* locus did not encode a delta-hemolysin-like peptide.

Conclusions:

  • *S. lugdunensis* possesses *agr*-like sequences and a related transcript.
  • Hemolytic activity in these strains is likely controlled by *agr-sl* but encoded elsewhere.
  • This suggests a divergent regulatory mechanism compared to *S. aureus*.