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Cholesterol side-chain cleavage by immobilized cells of Rhodococcus equi DSM 89-133
1Department of Microbiology, College of Basic Sciences and Humanities, G B Pant University of Agriculture and Technology, Pantnagar, India.
Indian Journal of Experimental Biology
|April 1, 1993
Abstract:
Side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of R. equi DSM 89-133 immobilized in polyacrylamide gel. In batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. Free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. Maximal activity of the immobilized cells was 60 to 70% of the free cells.