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Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae

R S Munson1, S Grass, R West

  • 1Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.

Infection and Immunity
|September 1, 1993
PubMed

Insights

Researchers identified the outer membrane protein P5 gene in Haemophilus influenzae. This protein shares similarities with Escherichia coli

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Haemophilus influenzae is a significant human pathogen.
  • Outer membrane proteins play crucial roles in bacterial structure and interaction.

Purpose of the Study:

  • To identify and characterize the gene encoding outer membrane protein P5 (P5) from Haemophilus influenzae serotype b.
  • To analyze the sequence and structural properties of the P5 protein.

Main Methods:

  • Genomic library screening using immunological methods.
  • Gene subcloning and expression analysis.
  • DNA sequencing of the P5 gene.
  • Molecular weight determination of the mature P5 protein.

Main Results:

  • The gene for outer membrane protein P5 was successfully identified from Haemophilus influenzae serotype b strain 1613.
  • Plasmid clones expressing P5 were confirmed through immunologic screening.
  • The mature P5 protein has a molecular weight of 35,628 Da.
  • Sequence analysis revealed 50% identity and 65% similarity to the OmpA protein of Escherichia coli.

Conclusions:

  • The P5 gene has been isolated and characterized.
  • The P5 protein exhibits structural homology to E. coli OmpA, suggesting potential functional similarities.

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