Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemically defined medium for Rothia dentocariosa

R J Lesher, S J Deal

    Applied and Environmental Microbiology
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Are doctors ignoring living wills?

    Pennsylvania medicine·1996
    Same author

    Outbreak of hypersensitivity pneumonitis in an industrial setting.

    JAMA·1988
    Same author

    Enteritis caused by Pasteurella pneumotropica infection in hamsters.

    Journal of clinical microbiology·1985
    Same author

    Comparison of fluorescent antibody, bacitracin susceptibility, latex agglutination, coagglutination, and API 20S for identifying group A streptococci.

    Canadian journal of microbiology·1985
    Same author

    Urease production from clinical isolates of beta-hemolytic Escherichia coli.

    Journal of clinical microbiology·1978
    Same author

    Levan production by a strain of Rothia: activation of complement resulting in cytotoxicity for human gingival cells.

    Journal of dental research·1977
    Same journal

    A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

    Applied and environmental microbiology·2026
    Same journal

    Sources and traits of bacteria and fungi found in the near-surface atmosphere.

    Applied and environmental microbiology·2026
    Same journal

    Advancing One Health in Africa through continental early warning environmental surveillance.

    Applied and environmental microbiology·2026
    Same journal

    Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

    Applied and environmental microbiology·2026
    Same journal

    Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

    Applied and environmental microbiology·2026
    Same journal

    Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

    Applied and environmental microbiology·2026
    See all related articles

    A new chemically defined medium supports the growth of Rothia dentocariosa. This medium allows for consistent bacterial generation times and high yields, crucial for further research.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Oral Microbiome

    Background:

    • Rothia dentocariosa is a significant component of the human oral microbiome.
    • Understanding its nutritional requirements is essential for studying its role in oral health and disease.
    • Previous culture methods lacked precise control over nutritional factors.

    Purpose of the Study:

    • To establish a chemically defined medium for Rothia dentocariosa.
    • To assess the growth characteristics of Rothia dentocariosa in this new medium.
    • To provide a reliable tool for future research on this bacterium.

    Main Methods:

    • Development and testing of a novel chemically defined culture medium.
    • Inoculation with multiple Rothia dentocariosa strains, including the type strain.

    Related Experiment Videos

  • Monitoring of growth parameters such as generation time and dry weight yield over 72 hours.

    • Main Results:

    • A chemically defined medium was successfully established for Rothia dentocariosa.
    • All tested Rothia strains exhibited robust growth in the defined medium.
    • Observed generation times ranged from 3.5 to 4.0 hours.
    • A consistent yield of 62 mg (dry weight) per 100 ml was achieved after 72 hours of growth.

    Conclusions:

    • The established chemically defined medium is effective for culturing Rothia dentocariosa.
    • This medium facilitates reproducible growth studies, enabling deeper investigation into Rothia dentocariosa biology.
    • The findings provide a foundation for future research into the ecological and pathogenic roles of Rothia dentocariosa.