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Improved specificity of testing methods for filovirus antibodies
L H Elliott1, S P Bauer, G Perez-Oronoz
1Division of Viral and Rickettsial Diseases, U.S. Department of Health and Human Services, Atlanta, GA 30333.
Journal of Virological Methods
|June 1, 1993
Summary
Improved filovirus antibody detection methods were developed due to an epizootic. Standardizing indirect fluorescent antibody assays (IFA) and optimizing Western blots (WB) enhanced sensitivity and specificity for filovirus testing.
Area of Science:
- Virology
- Immunology
- Public Health
Background:
- An epizootic involving imported monkeys necessitated rapid detection of filovirus antibodies.
- Existing diagnostic assays for filoviruses faced challenges with sensitivity and specificity.
- Thousands of samples required testing by the Centers for Disease Control and Prevention (CDC).
Purpose of the Study:
- To improve methods for detecting antibodies against filoviruses.
- To address limitations in sensitivity and specificity of current diagnostic assays.
Main Methods:
- Standardized protocols for indirect fluorescent antibody assays (IFA).
- Minimized antigen load in Western blot (WB) assays.
- Incorporated skim milk into diluents for Western blot optimization.
Main Results:
- Developed enhanced methods for detecting filovirus antibodies.
- Improved sensitivity and specificity in both IFA and WB assays.
- Standardization and antigen load reduction were key to assay improvement.
Conclusions:
- The study successfully improved filovirus antibody detection techniques.
- Optimized IFA and WB methods provide more reliable diagnostic capabilities.
- These advancements are crucial for managing future filovirus outbreaks.