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Paramyxoviruses and Paget's disease
E J Cartwright1, M T Gordon, A J Freemont
1Department of Cell and Structural Biology, University of Manchester, England.
Journal of Medical Virology
|June 1, 1993
Summary
Canine distemper virus (CDV) sequences were found in bone cells of Paget's disease patients, suggesting CDV may cause this human bone disorder. Viral presence was detected in both untreated and pamidronate-treated patients.
Area of Science:
- Virology
- Bone Biology
- Pathogen Discovery
Background:
- Paget's disease of bone is a chronic disorder characterized by abnormal bone remodeling.
- Previous studies suggested a potential viral etiology for Paget's disease.
Purpose of the Study:
- To investigate the presence of specific viral sequences, including canine distemper virus (CDV) and measles virus (MV), in bone samples from Paget's disease patients.
- To determine if treatment with pamidronate affects the presence of these viral sequences.
Main Methods:
- In situ hybridization technique was employed using 35S-labelled riboprobes.
- Bone samples from untreated and pamidronate-treated Paget's disease patients were analyzed.
- Detection of canine distemper nucleocapsid (CDV-N), measles nucleocapsid (MV-N), respiratory syncytial virus fusion (RSV-F), and simian virus 5 haemoglutinin-neuraminidase (SV5-HN) sequences.
Main Results:
- Canine distemper virus nucleocapsid (CDV-N) mRNA was detected in osteoclasts, osteoblasts, and mononuclear cells.
- CDV sequences were found in 63.5% of untreated Paget's patients and 48.2% of patients treated with pamidronate.
- Respiratory syncytial virus fusion (RSV-F) mRNA was detected in only one patient; other tested paramyxoviruses were not detected.
Conclusions:
- Canine distemper virus can infect human bone cells.
- CDV may be an etiological agent in the development of Paget's disease of bone.
- The presence of CDV sequences persists even after pamidronate treatment.