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[Detection of drug resistant-genes in cisplatin-resistant colon carcinoma cells by RT-PCR assay. II]
1Department of Clinical and Laboratory Medicine, Tohoku University School of Medicine, Sendai.
Abstract:
The c-fos, c-Ha-ras and c-myc oncogenes have been proposed to play an important role in DNA synthesis. Expression of c-fos, c-Ha-ras and c-myc genes were compared with the human colon carcinoma cell line HCT8S cells and a subline that was 4.5 resistant to cis-diamminedichloro-platinum (cisplatin). Resistant cells (HCT8DDP) exhibited a 4.0, 2.8 and 1.1 fold in mRNA for these genes when compared with the parent cells by Northern blotting analysis, respectively. The reverse transcription (RT)-PCR method has been demonstrated to quantify a sequence of these oncogenes. This method allowed the detection of gene expression from minimal cells. Thus RT-PCR assay could be an effective device in th early detection of oncogenes to cisplatin-resistance.
Insights
The study found that oncogenes c-fos, c-Ha-ras, and c-myc mRNA levels increase in cisplatin-resistant colon cancer cells. Reverse transcription PCR (RT-PCR) can detect these changes for early identification of cisplatin resistance.
Area of Science:
- Molecular biology
- Cancer genetics
- Oncogene research
Context:
- The role of oncogenes in DNA synthesis is crucial.
- Colon carcinoma cell lines provide a model for cancer research.
- Cisplatin resistance is a significant challenge in cancer therapy.
Purpose:
- To compare the expression of c-fos, c-Ha-ras, and c-myc oncogenes in cisplatin-sensitive and cisplatin-resistant human colon carcinoma cells.
- To evaluate the utility of the reverse transcription PCR (RT-PCR) method for detecting oncogene expression changes related to cisplatin resistance.
Summary:
- Expression of c-fos, c-Ha-ras, and c-myc oncogenes was significantly upregulated in cisplatin-resistant HCT8DDP cells compared to parent HCT8S cells.
- Northern blotting analysis revealed fold increases in mRNA for these oncogenes in resistant cells.
- Reverse transcription PCR (RT-PCR) proved effective in quantifying oncogene expression, even from minimal cell samples.
Impact:
- The findings suggest that increased expression of these oncogenes is associated with cisplatin resistance in colon cancer.
- RT-PCR assay shows potential as an early detection tool for identifying oncogenes linked to cisplatin resistance.
- This research contributes to understanding mechanisms of drug resistance and developing targeted therapies.
