Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Renal gene expression

J P Coghlan1, I A Darby, J D Penschow

  • 1Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.

Kidney International. Supplement
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of M1/M2 Tumour-Associated Macrophages (TAMs) and Th1/Th2 Cytokine Profiles in Patients with NSCLC.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society·2015
Same author

Processing and bioactivity of the corpuscles of Stannius protein of the Australian eel.

Fish physiology and biochemistry·2013
Same author

Aldosterone secretion: a molecular perspective.

Trends in endocrinology and metabolism: TEM·2008
Same author

Intracellular cyclic nucleotide analogues inhibit in vitro mitogenesis and activation of fibroblasts derived from obstructed rat kidneys.

Nephron. Experimental nephrology·2004
Same author

Quantitation of in situ hybridization using image analysis of radioactively labeled RNA probes.

Methods in molecular biology (Clifton, N.J.)·2001
Same author

Sox18 is transiently expressed during angiogenesis in granulation tissue of skin wounds with an identical expression pattern to Flk-1 mRNA.

Laboratory investigation; a journal of technical methods and pathology·2001

Hybridization histochemistry, using DNA probes, offers specific insights into kidney gene expression. This technique advances understanding of renal physiology and pathology for new diagnostic approaches.

Area of Science:

  • Molecular Biology
  • Histochemistry
  • Renal Science

Background:

  • Hybridization histochemistry utilizes cloned DNA probes for studying cells and tissues.
  • The kidney's complexity makes it a rewarding area for this technique.
  • Advancements in synthesized DNA oligonucleotides have accelerated probe development.

Purpose of the Study:

  • To highlight the potential of hybridization histochemistry in renal research.
  • To demonstrate its application in understanding normal renal physiology.
  • To showcase its utility in developing novel diagnostic approaches for renal pathology.

Main Methods:

  • Manufacturing and purification of specific synthetic DNA probes (oligonucleotides).
  • Labeling of probes for detection.

Related Experiment Videos

  • Application of probes to determine gene expression in specific kidney cells.

    • Main Results:

    • Demonstrated the specificity of DNA probes in complex tissues like the kidney.
    • Showcased the rapid development and application of synthetic probes.
    • Provided examples of insights into renal gene expression.

    Conclusions:

    • Hybridization histochemistry with synthetic DNA probes offers unparalleled specificity for studying renal physiology and pathology.
    • The technique provides unique insights into cellular gene expression.
    • It holds significant potential for advancing diagnostics in nephrology.