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Structural analysis of the human ret proto-oncogene using exon trapping
J B Kwok1, E Gardner, J P Warner
1Department of Pathology, University of Cambridge.
Oncogene
|September 1, 1993
Summary
Researchers mapped the human ret proto-oncogene, identifying at least 20 exons spanning 30 kb of genomic DNA. This detailed genomic structure provides insight into ret gene organization and function.
Area of Science:
- Genomics
- Molecular Biology
- Oncogenes
Background:
- The ret proto-oncogene plays a crucial role in neural crest development and is implicated in various human cancers.
- Understanding the genomic organization of ret is essential for investigating its function and dysregulation.
Purpose of the Study:
- To construct a detailed genomic map of the human ret proto-oncogene.
- To delineate the exon-intron structure and physical organization of the ret gene.
Main Methods:
- Isolation and assembly of overlapping cosmid clones to create a genomic contig.
- Exon trapping and sequencing to identify ret exons and their boundaries.
- Restriction mapping and PCR-based methods for precise exon localization on genomic DNA.
Main Results:
- A genomic contig of the human ret proto-oncogene was established.
- At least 20 exons, ranging from 60 bp to 287 bp, were identified within approximately 30 kb of genomic DNA.
- The extracellular, transmembrane, and cytoplasmic domains are encoded by 10, 1, and 9 exons, respectively.
Conclusions:
- The study provides a comprehensive genomic map of the human ret proto-oncogene.
- This detailed structural information is fundamental for future studies on ret gene regulation, function, and its role in disease.