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A cytotoxicity test for the detection of Campylobacter jejuni toxin
1Department of Epidemiology and Preventive Medicine, University of California, Davis 95616.
Veterinary Microbiology
|May 1, 1993
Abstract:
A 7-h 51Cr-release cytotoxicity assay has been developed to detect the presence of Campylobacter jejuni toxin in the culture supernatant. Some of the culture supernatants have cytotoxic effects against labeled chicken lymphocytes, while some others do not have this effect. There is no direct correlation between the pathogenicity of the organisms and the presence of the toxin. The supernatant of C. jejuni retains its cytotoxicity after heating at 100 degrees C for 10 min.; therefore, it is a heat stable toxin.