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Nucleotide sequence of the rabbit gamma-preprotachykinin I cDNA
H J Mägert1, A Heitland, M Rose
1Lower Saxony Institute for Peptide-Research, Hannover, Germany.
Biochemical and Biophysical Research Communications
|August 31, 1993
Abstract:
We succeeded in amplifying tachykinin I specific cDNAs from cerebral tissue of guinea pig, rabbit, rat, hamster, trout and tupaia in the expected size range of 820-980 bp. The amplified 859 bp rabbit gamma-preprotachykinin I cDNA was sequenced and consisted of the whole 345 bp gamma-PPT I coding sequence including the substance P and neurokinin A coding regions and a 505 bp large 3'-nontranslated region. Both, molecular weight and sequence comparison emphasizes the very high phylogenetic age of the preprotachykinin I gene.