A series of vectors that simplify mammalian gene targeting

Related Experiment Video

Updated: Nov 7, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

A J Brookes1, B J Stevenson, D J Porteous

  • 1MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.

Transgenic Research
|July 1, 1993
PubMed

More Related Videos

Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord08:39

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Published on: March 18, 2013

Related Concept Videos

In-vitro Mutagenesis (Gene Knockouts)02:29

In-vitro Mutagenesis (Gene Knockouts)

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...