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Related Experiment Videos

Methionine-mediated lethality in yeast cells at elevated temperature

H Jakubowski1, E Goldman

  • 1Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103.

Journal of Bacteriology
|September 1, 1993
PubMed
Summary

Methionine causes yeast cells to lose viability at high temperatures. This lethality is linked to the inability to synthesize adenosine 5'-phosphosulfate (APS), a key molecule for thermotolerance.

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Area of Science:

  • * Molecular Biology
  • * Cellular Stress Response
  • * Biochemistry

Background:

  • * Saccharomyces cerevisiae (baker's yeast) exhibits temperature-dependent viability.
  • * Methionine's role in cellular stress, particularly at elevated temperatures, is not fully understood.
  • * The sulfate assimilation pathway is crucial for cellular metabolism.

Purpose of the Study:

  • * To investigate the mechanism of methionine-mediated lethality in yeast at high temperatures.
  • * To determine the role of sulfate assimilation intermediates in yeast thermotolerance.
  • * To elucidate the link between methionine, APS synthesis, and survival under heat stress.

Main Methods:

  • * Comparative analysis of Saccharomyces cerevisiae viability under different growth conditions (presence/absence of methionine) and temperature shifts.

Related Experiment Videos

  • * Measurement of intracellular levels of sulfate assimilation pathway intermediates, specifically adenosine 5"-phosphosulfate (APS) and adenosine 5"-phosphosulfate 3"-phosphate.
  • * Genetic analysis involving yeast strains with repressed or mutated MET3 gene, affecting APS synthesis.

    • Main Results:

    • * Yeast grown in methionine-containing media lost viability when shifted to 45°C, while those grown without methionine survived.
    • * Elevated temperatures induced a posttranslational increase in APS and adenosine 5"-phosphosulfate 3"-phosphate levels in methionine-absent cultures.
    • * Yeast strains unable to synthesize APS (due to methionine repression or MET3 mutation) did not survive the temperature increase.

    Conclusions:

    • * Methionine-mediated lethality at elevated temperatures in Saccharomyces cerevisiae is directly associated with impaired APS synthesis.
    • * Adenosine 5"-phosphosulfate (APS) plays a critical role in yeast thermotolerance.
    • * The sulfate assimilation pathway is a significant factor in cellular adaptation to heat stress.