Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Crystals of a hammerhead ribozyme

H W Pley1, D S Lindes, C DeLuca-Flaherty

  • 1Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, California 94305.

The Journal of Biological Chemistry
|September 15, 1993
PubMed
Summary

Researchers crystallized hammerhead ribozyme-inhibitor complexes, revealing a promising crystal form for detailed structure determination. This advancement aids in understanding RNA enzyme mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous NOD1 and NOD2 Deletion in Hematopoietic Stem and Progenitor cells Promotes Long-Term Donor Chimerism.

Transplantation and cellular therapy·2026
Same author

Molecular strategies used by hibernators: Potential therapeutic directions for ischemia reperfusion injury and preservation of human donor organs.

Transplantation reviews (Orlando, Fla.)·2019
Same author

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay.

Veterinary record open·2016
Same author

The emergence of Leptospira borgpetersenii serovar Arborea as the dominant infecting serovar following the summer of natural disasters in Queensland, Australia 2011.

Tropical biomedicine·2014
Same author

Neutrophil counts in leptospirosis patients infected with different serovars.

Tropical biomedicine·2014
Same author

Emerging tropical diseases in Australia. Part 1. Leptospirosis.

Annals of tropical medicine and parasitology·2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Hammerhead ribozymes are catalytic RNA molecules involved in gene regulation.
  • Understanding their structure is crucial for developing therapeutic inhibitors.
  • Crystallization is a key technique for determining high-resolution molecular structures.

Purpose of the Study:

  • To crystallize hammerhead ribozyme-inhibitor complexes.
  • To identify crystal forms suitable for detailed structural analysis.
  • To facilitate structure-based drug design targeting ribozyme activity.

Main Methods:

  • Co-crystallization of RNA enzyme strands and DNA inhibitor strands.
  • Screening of multiple crystallization conditions.
  • X-ray diffraction analysis of obtained crystal forms.

Main Results:

  • Four distinct crystal forms of the hammerhead ribozyme-inhibitor complex were obtained.
  • One crystal form exhibits promising diffraction properties (approx. 3.2 A resolution).
  • This optimal crystal form belongs to the trigonal space group P3(1)21 (or its enantiomorph) with specific unit cell dimensions (a = 92.5 A, c = 185.0 A).

Conclusions:

  • Successful crystallization of hammerhead ribozyme-inhibitor complexes has been achieved.
  • A specific trigonal crystal form is identified as highly suitable for high-resolution structure determination.
  • These findings pave the way for elucidating the precise three-dimensional structure of these complexes.

Related Experiment Videos