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Related Experiment Videos

Albumin and Ricinus communis agglutinin decrease endothelial permeability via interactions with matrix

R Qiao1, A Siflinger-Birnboim, H Lum

  • 1Department of Physiology and Cell Biology, Albany Medical College, New York 12208.

The American Journal of Physiology
|August 1, 1993
PubMed
Summary
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Albumin and Ricinus communis agglutinin (RCA) significantly reduce hydraulic conductivity (Lp) in bovine pulmonary microvascular endothelial cell monolayers. RCA

Area of Science:

  • Endothelial cell biology
  • Transport phenomena
  • Biochemistry

Background:

  • Albumin and RCA are known to affect endothelial permeability.
  • Understanding their impact on hydraulic conductivity is crucial for vascular research.

Purpose of the Study:

  • To investigate the effects of albumin and RCA on hydraulic conductivity (Lp) of bovine pulmonary microvascular endothelial cell monolayers (BPMVEC).
  • To determine the role of extracellular matrix (ECM) in these effects.

Main Methods:

  • BPMVEC monolayers were cultured on microporous filters.
  • Liquid flux was measured at varying hydrostatic pressures to calculate Lp.
  • Effects of albumin and RCA were assessed with and without D-galactose and on isolated ECM.

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Main Results:

  • Both albumin and RCA significantly decreased total Lp.
  • The effect of RCA was reversible with D-galactose, indicating a specific lectin-carbohydrate interaction.
  • Both agents also reduced Lp of the extracellular matrix, but not the filter's Lp.

Conclusions:

  • Albumin and RCA impair endothelial cell hydraulic conductivity.
  • RCA's effect is mediated by specific carbohydrate binding.
  • The extracellular matrix plays a significant role in the observed changes in Lp.