Effects of long-term restricted feeding on motor activity rhythm in the rat

The motor activity circadian rhythm of the rat is driven by at least two different pacemakers, one entrained by light-dark cycles and the other by periodic food access. The interaction between these two pacemakers generates the overt rhythm in many variables in the rat. In this paper we study the effects of restricted feeding (RF) for > 300 days on the motor activity of two groups of eight rats each (4 males and 4 females) that were kept under different light patterns. The first group was kept under dim red light, and the second group was kept initially for 70 days under bright light and afterward under dim red light. Rats had access to food for only 2 h/day. Results show that strong alterations in the motor activity pattern, such as relative coordination, bouncing, and several cases of entrainment, are shown in the first group. When these animals return to ad libitum feeding conditions the presence of the food component is clear, in some cases for > 20 days. In contrast, the rats that were previously subjected to bright light did not entrain to RF. In this group, there was a masking effect when lights were on, but a free-running component appeared when lights were off. The endogenous period (tau) of this component was not modified during the course of the experiment. After RF these rats showed a longer tau than the former group.(ABSTRACT TRUNCATED AT 250 WORDS)