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Related Experiment Videos

Interactions between denture lining material, protein pellicles and Candida albicans

H Nikawa1, S Hayashi, Y Nikawa

  • 1Department of Prosthetic Dentistry, Hiroshima University School of Dentistry, Japan.

Archives of Oral Biology
|July 1, 1993
PubMed
Summary

Saliva and serum pellicles on denture lining materials significantly increase Candida albicans colonization and hyphal invasion. This suggests these pellicles may potentiate yeast growth on oral appliances.

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Area of Science:

  • Oral microbiology
  • Biomaterials science
  • Mycology

Background:

  • Denture stomatitis, often caused by Candida albicans, affects many individuals using oral appliances.
  • Pellicles, protein-rich layers, form on oral surfaces and biomaterials, influencing microbial interactions.
  • The role of specific salivary components in Candida albicans adhesion to denture lining materials requires further elucidation.

Purpose of the Study:

  • To investigate the influence of different pellicle compositions (saliva, serum, mucin, lysozyme) on Candida albicans colonization of tissue conditioner lining material.
  • To assess the impact of pellicle coating on pH changes and yeast adherence to the lining material.
  • To examine the ultrastructural changes and hyphal invasion of Candida albicans on coated and uncoated lining materials.

Main Methods:

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  • Tissue conditioner lining materials were coated with saliva, serum, mucin, or lysozyme, or left uncoated (control).
  • pH changes in culture media were monitored over 120 hours of incubation with Candida albicans.
  • Scanning electron microscopy (SEM) was used to evaluate yeast colonization and morphology on the coated and uncoated materials.

Main Results:

  • No significant differences in pH reduction were observed between control and coated lining materials after 120 hours.
  • SEM revealed significantly greater Candida albicans colonization on saliva- and serum-coated lining materials compared to lysozyme-, mucin-coated, or control materials.
  • Hyphal invasion by Candida albicans was observed on saliva-coated lining materials.

Conclusions:

  • Denture pellicles derived from saliva and serum can enhance Candida albicans colonization on tissue conditioner lining materials.
  • These findings suggest that salivary and serum components in pellicles may promote candidal adhesion and biofilm formation on oral appliances.
  • Understanding these interactions is crucial for developing strategies to prevent Candida-associated denture stomatitis.