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Related Experiment Videos

A permanent cell viability assay using alcian blue

J T Thornthwaite, R C Leif

    Stain Technology
    |July 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Alcian blue dye exclusion with centrifugal cytology offers a method to assess cell viability in fixed cell suspensions. This technique revealed distinct membrane changes in nonviable cells versus the uniform surfaces of viable cells.

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    Area of Science:

    • Cell biology
    • Cytology
    • Biomedical imaging

    Background:

    • Assessing cell viability is crucial in biological research.
    • Glutaraldehyde fixation is commonly used for preserving cellular structures.
    • Existing viability assays may have limitations for fixed cell suspensions.

    Purpose of the Study:

    • To evaluate the efficacy of the alcian blue dye exclusion method combined with centrifugal cytology for assessing glutaraldehyde-fixed cell viability.
    • To characterize the morphological differences between viable and nonviable cells using this combined technique.

    Main Methods:

    • Utilized alcian blue dye exclusion method on glutaraldehyde-fixed cells.
    • Employed centrifugal cytology for sample preparation.
    • Separated spleen cell suspensions using linear bovine serum albumin density gradient centrifugation.

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  • Performed combined light and scanning electron microscopy for detailed cell analysis.

    • Main Results:

    • The alcian blue dye exclusion method successfully prepared permanent records of cell viability.
    • Nonviable cells stained with alcian blue exhibited membrane alterations.
    • Viable cells remained spherical with uniform surface features.
    • Density gradient centrifugation effectively separated spleen cell suspensions.

    Conclusions:

    • The combined alcian blue dye exclusion and centrifugal cytology method is effective for determining cell viability in fixed cell suspensions.
    • Distinct morphological differences observed via microscopy aid in distinguishing viable from nonviable cells.
    • This method provides a reliable approach for creating permanent records of cell viability.