Purification of rat intestinal receptor for intrinsic factor-vitamin B-12 complex by affinity chromatography

Rat intrinsic factor was bound to vitamin B-12-Sepharose to produce intrinsic factor-vitamin B-12-Sepharose. Intestinal receptor for intrinsic factor-vitamin B-12 complex was purified from rat ileal extract by affinity chromatography using the intrinsic factor-vitamin B-12-Sepharose as an affinity adsorbent with recovery of 48.5% and specific activity increased 335 fold of original sample.