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Updated: Aug 12, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
Asymmetric charge distributions in planar bilayer systems
Abstract:
Using the simple argument based on irreversible thermodynamics and the Gouy-Chapman theory of the double layer, we show that the equilibrium distribution of charged lipid molecules between the two surfaces of a bilayer is asymmetric if the two solutions bathing the surfaces have the same ionic strength but contain ions of different valencies. For example, if one bathing solution contains 0.10 M NaCl and the other contains 0.70 M NaCl and 0.10 M CaCl2, the ratio of charged lipid molecules of the two surfaces in a membrane that contains 50% total negative lipids is 1.46, leading to a transbilayer potential of 18 mV. A complete set of such numerical results is presented in four figures.
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