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Related Experiment Videos

How does a virus bud?

D M Lerner1, J M Deutsch, G F Oster

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Biophysical Journal
|July 1, 1993
PubMed
Summary
This summary is machine-generated.

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Viral budding from host plasma membranes is not driven by membrane fluctuations alone. Glycoprotein spike diffusion may limit budding rates, suggesting alternative mechanisms are involved in virus release.

Area of Science:

  • Biophysics
  • Virology
  • Cell Biology

Background:

  • Viruses utilize host cell machinery for replication and release.
  • Viral budding involves the egress of progeny virions from the host cell plasma membrane.

Purpose of the Study:

  • Investigate potential rate-limiting steps in viral budding from the plasma membrane.
  • Determine the contribution of membrane fluctuations, hydrodynamic interactions, and glycoprotein diffusion to budding kinetics.

Main Methods:

  • Computational modeling of plasma membrane mechanics.
  • Analysis of thermal fluctuations and bending moduli.
  • Kinetics modeling of glycoprotein diffusion to the budding site.

Main Results:

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  • Plasma membrane thermal fluctuations are insufficient to drive viral capsid wrapping for bending moduli > 3 x 10(-13) ergs.
  • Budding times of 10-20 minutes are consistent with diffusion-limited glycoprotein spike assembly.
  • Budding is more likely in regions of high membrane curvature.

    • Conclusions:

    • Viral budding likely involves mechanical forces beyond membrane thermal fluctuations.
    • Glycoprotein spike diffusion is a plausible rate-limiting factor for viral egress.
    • Membrane curvature may play a significant role in facilitating viral budding.