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Published on: March 17, 2014
Prevalence and biochemical properties of V factor-dependent Pasteurellaceae from rodents
1Central Animal Laboratories, German Cancer Research Centre, Heidelberg.
Abstract:
Organs of 1436 rats and 2839 mice were monitored for V-factor dependent Pasteurellaceae. Such bacteria were isolated from 21.2% of rats and from 2.4% of mice, respectively. They were most frequently cultured from the deep respiratory tract (lungs and trachea). In addition, they were found in the nasal cavity and on mucous membranes of the genital and the intestinal tract. 35 phenotypical criteria were determined for 429 isolates. Based on these data, groups of bacteria showing similar biochemical properties were formed using exploratory statistical methods (principal qualitative analysis). The majority of 333 isolates is represented in a rather homogenous group which has some characteristics in common with the Haemophilus parainfluenzae complex. The remaining isolates consist of several groups. It was shown by others that some of these bacteria are closely related to the yet unnamed Taxon B which forms a genus-like cluster together with Pasteurella pneumotropica type Jawetz.
Insights
V-factor dependent Pasteurellaceae were found in over 20% of rats and 2% of mice, primarily in the respiratory tract. Most isolates shared traits with Haemophilus parainfluenzae, with others related to Taxon B.
Area of Science:
- Microbiology
- Bacteriology
- Animal Health
Background:
- V-factor dependent Pasteurellaceae are bacteria requiring specific growth factors.
- Understanding the prevalence and characteristics of these bacteria in animal populations is crucial for diagnostics and disease management.
Purpose of the Study:
- To investigate the occurrence and phenotypic characteristics of V-factor dependent Pasteurellaceae in rats and mice.
- To classify isolated bacteria based on their biochemical properties.
Main Methods:
- Organs from 1436 rats and 2839 mice were cultured for V-factor dependent Pasteurellaceae.
- 35 phenotypic criteria were determined for 429 isolates.
- Exploratory statistical methods, including principal qualitative analysis, were used for bacterial grouping.
Main Results:
- Bacteria were isolated from 21.2% of rats and 2.4% of mice, predominantly from the respiratory tract.
- A large, homogenous group of 333 isolates exhibited characteristics similar to the Haemophilus parainfluenzae complex.
- Other isolates were grouped and found to be related to Taxon B and Pasteurella pneumotropica.
Conclusions:
- V-factor dependent Pasteurellaceae are commonly found in rats, particularly in the respiratory system.
- The study identified distinct phenotypic groups within these isolates, with implications for bacterial taxonomy and identification.
- Further research may clarify the taxonomic position of these isolates, including their relationship to Taxon B.
