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Detection of tetracycline resistance protein encoded by Bacillus subtilis plasmid pNS1
1Department of Biochemistry, Faculty of Pharmaceutical Sciences, Higashi-Nippon-Gakuen University, Hokkaido, Japan.
Biological & Pharmaceutical Bulletin
|January 1, 1993
Abstract:
Tetracycline resistance protein (TET) of Bacillus subtilis plasmid pNS1 was detected by immunoblot analysis using a specific antibody to TET-chloramphenicol acetyltransferase (CAT) fusion protein. In two-dimensional electrophoresis, one major spot which seemed to be the pNS1-encoded TET (pNS1-TET), was detected by immunostaining. Its molecular weight and isoelectric point were approximately 52 kDa and 6.2, respectively. Judging from the nucleotide sequence, the pNS1-TET is a very hydrophobic, 50 kDa protein. Therefore, the 52 kDa protein is thought to be an intact form of the pNS1-TET produced by B. subtilis cells.