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Bidirectional modulation of human B cell stimulation by activated T cells
12nd Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Cellular Immunology
|September 1, 1993
Summary
Human T cells can simultaneously help and suppress B cell responses. Activated T cells provide help to resting B cells while suppressing preactivated B cells, with B cell activation state influencing T cell function.
Area of Science:
- Immunology
- Cellular Immunology
- T cell-B cell interactions
Background:
- Human T cells exhibit both helper and suppressor functions towards B cells.
- The precise mechanisms and outcomes of these dual T cell effects on B cells remain unclear.
Purpose of the Study:
- To investigate the detailed functions of human peripheral blood T cells activated by immobilized anti-CD3.
- To elucidate how T cell activation duration and phenotype modulate B cell responses, including help and suppression.
Main Methods:
- Human CD4+ and CD8+ T cell subsets (CD45RA+ or CD45RA-) were activated with immobilized anti-CD3 for up to 20 days.
- Activated T cells were co-cultured with resting or preactivated B cells at various time points.
- B cell activation markers (CD71, CD25) and maturation were assessed; effects of mitomycin C and IL-2 were evaluated.
Main Results:
- Activated CD4+ and CD8+ T cells provided help to resting B cells, indicated by CD71 and CD25 expression, up to 17 days post-activation.
- T cell help potency decreased for CD8+ subsets after 17 days.
- Simultaneously, activated T cells suppressed the maturation of preactivated B cells; this suppression was abrogated by mitomycin C treatment up to 13 days, but not by exogenous IL-2.
Conclusions:
- Human activated T cells can exert simultaneous help and suppression on B cells, independent of T cell phenotype.
- The activation state of B cells appears critical in determining the functional outcome of T cell-B cell interactions.