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Analysis of hapten-carrier protein conjugates by nondenaturing gel electrophoresis
C Kamps-Holtzapple1, R J Carlin, C Sheffield
1Food Animal Protection Research Laboratory, U.S. Department of Agriculture, College Station, TX 77845.
Abstract:
Hapten-carrier protein conjugates were made using five different small haptens (MW < 1000), two carrier proteins and two methods of conjugation. Nondenaturing agarose gel electrophoresis was used to demonstrate that when as few as two molecules of a small hapten are attached to the carrier, the conjugate band migrates differently from that of the carrier alone or of the coupling reagent-treated carrier. Furthermore, the direction of the change in migration of each conjugate correlates with the change in charge which occurs upon attachment of the hapten to the carrier.
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