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Related Experiment Videos

CD68 positive epidermal dendritic cells

P Petzelbauer1, D Födinger, K Rappersberger

  • 1Department of Dermatology, University of Vienna Medical School, Austria.

The Journal of Investigative Dermatology
|September 1, 1993
PubMed
Summary
This summary is machine-generated.

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Pemphigus foliaceus (PF) skin lesions show a shift from CD1a+ Langerhans cells to CD1a- dendritic cells expressing CD68. These CD68+ cells may represent transitional myelomonocytic cells transforming into Langerhans cells in PF.

Area of Science:

  • Immunodermatology
  • Cell Biology
  • Autoimmune Diseases

Background:

  • Pemphigus foliaceus (PF) is characterized by intraepidermal blistering.
  • Previous studies noted an increase in CD68+ dendritic cells in PF epidermis.
  • The nature of this CD68+ cell population in PF remains unclear.

Purpose of the Study:

  • To investigate the phenotype and origin of intraepidermal CD68+ dendritic cells in pemphigus foliaceus.
  • To compare these cells with those found in pemphigus vulgaris (PV).

Main Methods:

  • Analysis of lesional skin from five PF patients and comparison with PV patients.
  • Utilized a panel of monoclonal antibodies with immunoperoxidase and immunofluorescence techniques.
  • Immunoelectron microscopy was employed to examine cellular ultrastructure and Birbeck granules (BG).

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Main Results:

  • PF epidermis showed significantly fewer CD1a+ Langerhans cells compared to PV.
  • PF epidermis contained abundant bone marrow-derived (CD45+) cells expressing CD68, HLA-DR, CD11c, and CD18.
  • These CD68+ cells were predominantly CD1a- and lacked BG, but a subset was CD1a+ with BG, suggesting a transitional form.

Conclusions:

  • A shift occurs in PF from CD1a+/CD68- Langerhans cells to CD1a-/CD68+ dendritic cells.
  • The presence of CD1a+/CD68+/BG+ cells suggests a link between Langerhans cells and CD68+ cells.
  • These findings indicate CD68+ dendritic cells in PF may be transitional myelomonocytic cells differentiating into epidermal Langerhans cells.