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Estimation of gold-labelled macromolecules attached to cell surfaces

N Thomas1, I ap Gwynn

  • 1Department of Biological Sciences, University of Wales, Aberystwyth.

Journal of Microscopy
|June 1, 1993
PubMed

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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.