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Related Experiment Videos

Free hormone assays

R Ekins1

  • 1Department of Molecular Endocrinology, University College London Medical School, UK.

Nuclear Medicine Communications
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Serum proteins bind thyroid and steroid hormones, but their physiological role remains unclear. Current free hormone assays often contradict basic physicochemical principles, leading to confusion in endocrinology.

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Area of Science:

  • Endocrinology and Physiology
  • Biochemistry
  • Physical Chemistry

Background:

  • The physiological significance of serum protein binding for thyroid and steroid hormones is not fully understood.
  • Disagreements persist regarding the physicochemical implications of these proteins in the microcirculation.
  • Theoretical analysis of protein binding effects on hormone uptake in tissues is complex and incomplete.

Purpose of the Study:

  • To analyze the physicochemical consequences of hormone binding in vivo.
  • To provide a basis for validating the free hormone hypothesis.
  • To clarify the physiological role of serum binding proteins.

Main Methods:

  • Theoretical analysis of intracapillary protein binding reactions.
  • Examination of physicochemical principles underlying free hormone measurement techniques.

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  • Critical review of immunoassay kits and their underlying concepts.

    • Main Results:

    • The exact role of serum binding proteins in hormone transport and physiological function requires further elucidation.
    • Many widely used 'free hormone' immunoassay kits are based on flawed physicochemical principles.
    • Commercial kits often use empirically adjusted reagent cocktails, leading to confusion and the propagation of incorrect concepts.

    Conclusions:

    • A comprehensive physicochemical analysis of in vivo hormone binding is necessary.
    • The validity of the free hormone hypothesis and the role of binding proteins remain open questions.
    • Current free hormone assay methodologies require critical re-evaluation due to fundamental physicochemical inaccuracies.