Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Free hormone assays

R Ekins1

  • 1Department of Molecular Endocrinology, University College London Medical School, UK.

Nuclear Medicine Communications
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Serum proteins bind thyroid and steroid hormones, but their physiological role remains unclear. Current free hormone assays often contradict basic physicochemical principles, leading to confusion in endocrinology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Responses to Colette Chiland's 'the psychoanalyst and the transsexual patient'.

The International journal of psycho-analysis·2001
Same author

Thyroid hormone receptor gene expression in first trimester human fetal brain.

The Journal of clinical endocrinology and metabolism·2000
Same author

Microarrays: their origins and applications.

Trends in biotechnology·1999
Same author

The role of the dental nurse in general practice.

British dental journal·1999
Same author

On the meaning of "sensitivity": a rejoinder.

Clinical chemistry·1998
Same author

On the meaning of "sensitivity".

Clinical chemistry·1997
Same journal

Diagnostic performance and quantitative characterization of PET/computed tomography in peritoneal deposits assessment.

Nuclear medicine communications·2026
Same journal

Diagnostic value of dual-time-point 18F-fluorodeoxyglucose PET/computed tomography in differentiating benign and malignant extrahepatic bile duct occupying lesions.

Nuclear medicine communications·2026
Same journal

Prognostic value of baseline 18F- fluroodexoygloucose PET/computed tomography-derived maximum standardized uptake value and tumor bulk in pediatric Hodgkin lymphoma: a retrospective analysis.

Nuclear medicine communications·2026
Same journal

A comparative assessment of [99mTc] HYNIC-PSMA-11 scintigraphy in prostate cancer as a potential substitute for disease evaluation with [68Ga] PSMA-11 PET/CT imaging.

Nuclear medicine communications·2026
Same journal

Prediction of tumor radiation absorbed dose in radionuclide therapy based on the mean standardized uptake value from PET imaging.

Nuclear medicine communications·2026
Same journal

Serum cytokine profiles in papillary thyroid carcinoma patients with lung metastases: characteristics and the relationship with prognosis.

Nuclear medicine communications·2026
See all related articles

Area of Science:

  • Endocrinology and Physiology
  • Biochemistry
  • Physical Chemistry

Background:

  • The physiological significance of serum protein binding for thyroid and steroid hormones is not fully understood.
  • Disagreements persist regarding the physicochemical implications of these proteins in the microcirculation.
  • Theoretical analysis of protein binding effects on hormone uptake in tissues is complex and incomplete.

Purpose of the Study:

  • To analyze the physicochemical consequences of hormone binding in vivo.
  • To provide a basis for validating the free hormone hypothesis.
  • To clarify the physiological role of serum binding proteins.

Main Methods:

  • Theoretical analysis of intracapillary protein binding reactions.
  • Examination of physicochemical principles underlying free hormone measurement techniques.

Related Experiment Videos

  • Critical review of immunoassay kits and their underlying concepts.

    • Main Results:

    • The exact role of serum binding proteins in hormone transport and physiological function requires further elucidation.
    • Many widely used 'free hormone' immunoassay kits are based on flawed physicochemical principles.
    • Commercial kits often use empirically adjusted reagent cocktails, leading to confusion and the propagation of incorrect concepts.

    Conclusions:

    • A comprehensive physicochemical analysis of in vivo hormone binding is necessary.
    • The validity of the free hormone hypothesis and the role of binding proteins remain open questions.
    • Current free hormone assay methodologies require critical re-evaluation due to fundamental physicochemical inaccuracies.