Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid C cells in the DiGeorge anomaly: a quantitative study

S Pueblitz1, A G Weinberg, J Albores-Saavedra

  • 1Department of Pathology, Children's Medical Center of Dallas, Texas.

Pediatric Pathology
|July 1, 1993
PubMed
Summary

DiGeorge anomaly (DGA) patients have fewer thyroid C cells than controls, confirming neural crest origin but suggesting potential endodermal contribution. This impacts understanding of DGA developmental defects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

H2A.Z overexpression suppresses senescence and chemosensitivity in pancreatic ductal adenocarcinoma.

Oncogene·2021
Same author

Hyaline vascular Castleman's disease involving the biliary tract.

Irish medical journal·2013
Same author

[Endoscopic submucosal dissection of a colonic tubulovillous adenoma with high grade dysplasia].

Revista de gastroenterologia de Mexico·2011
Same author

[Pseudohyperplastic carcinoma with xanthomatous changes: a neoplasm mimicking glandular hyperplasia of the prostate].

Actas urologicas espanolas·2010
Same author

Primary peritoneal mesotheliomas in children: a clinicopathological and immunohistochemical study of eight cases.

Histopathology·2008
Same author

Genetic analysis of high altitude paragangliomas.

Endocrine pathology·2006

Area of Science:

  • Developmental Biology
  • Endocrinology
  • Human Genetics

Background:

  • DiGeorge anomaly (DGA) involves developmental defects affecting branchial pouch derivatives, including neural crest cells.
  • Thyroid C cells, responsible for calcitonin production, are believed to originate from cephalic neural crest cells.
  • The ultimobranchial body is a key structure implicated in C cell development in humans.

Purpose of the Study:

  • To investigate the presence and quantity of thyroid C cells in children with DiGeorge anomaly.
  • To evaluate the potential impact of DGA on the development of C cells.
  • To explore the cellular origins of thyroid C cells in the context of DGA.

Main Methods:

  • Thyroid tissue sections from 16 DGA patients (incomplete and complete forms) and 16 age-matched controls were analyzed.

Related Experiment Videos

  • Immunoperoxidase staining was used to detect calcitonin and chromogranin, markers for C cells.
  • Quantitative analysis of C cells per high-power field (HPF) was performed.

    • Main Results:

    • Thyroid C cells were detected in 69% of DGA cases, a higher frequency than anticipated.
    • DGA patients exhibited significantly fewer C cells per HPF (1.6) compared to controls (4.9) (P < .005).
    • Both complete and incomplete DGA forms showed C cell deficiency, with incomplete cases having similar C cell numbers to complete cases.

    Conclusions:

    • Thyroid C cells are present in DGA patients, supporting their neural crest origin but in deficient numbers.
    • The findings confirm a developmental deficiency of C cells in DGA.
    • The data suggest a possible additional source of C cells, potentially from thyroid endoderm, analogous to gut endocrine cells.