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Updated: Aug 6, 2026

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
Published on: December 21, 2017
Alternative labeling techniques for automated fluorescence based analysis of PCR products
1Applied Biosystems, Division of Perkin-Elmer, Foster City, CA 94404.
Abstract:
We present here convenient and simple methods that utilize two new fluorescent tags, TOTO-1 and fluorescein-12-dUTP, in conjunction with analysis of PCR products on automated, fluorescence-based electrophoretic instruments. These methods should prove valuable in those applications wherein the effort or expense of covalently attaching a fluorescent tag onto one or both of the PCR primers is not justified. Advantages and disadvantages of the new labeling methods are then reviewed.
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