Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated fluorescence-based restriction fragment analysis

J E Lamerdin1, A V Carrano

  • 1Human Genome Center, Lawrence Livermore National Laboratory, CA 94550.

Biotechniques
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence-specific identification of 18 pathogenic microorganisms using microarray technology.

Molecular and cellular probes·2002
Same author

Restoration of nucleotide excision repair in a helicase-deficient XPD mutant from intragenic suppression by a trichothiodystrophy mutation.

Molecular and cellular biology·2001
Same author

Comparative maps of human 19p13.3 and mouse chromosome 10 allow identification of sequences at evolutionary breakpoints.

Genome research·2000
Same author

Characterization of the mouse Xpf DNA repair gene and differential expression during spermatogenesis.

Genomics·2000
Same author

Complex beta-satellite repeat structures and the expansion of the zinc finger gene cluster in 19p12.

Genome research·1998
Same author

High-resolution cartography of recently integrated human chromosome 19-specific Alu fossils.

Journal of molecular biology·1998

This study introduces an automated method for analyzing DNA restriction fragments using fluorescent labeling and gel electrophoresis. This technique enhances speed and efficiency in DNA fragment analysis for various molecular biology applications.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Automated analysis of DNA restriction fragments is crucial for genomic research.
  • Traditional methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop and describe a novel procedure for automated DNA restriction fragment analysis.
  • To showcase the efficiency and unique applications of this new method.

Main Methods:

  • Simultaneous digestion of DNA with restriction enzymes and ligation of a fluorochrome-labeled linker.
  • Electrophoresis and data analysis using a horizontal agarose gel scanning system (Applied Biosystems Model 362 GENE SCANNER).

Main Results:

  • Detailed description of mobility parameters and detection sensitivity.

Related Experiment Videos

  • Demonstrated rapid analysis of up to 72 cosmid restriction digests in 4 hours.
  • Showcased applicability for DNA transfers, hybridizations, cosmid mapping, and double-digest fragment end discrimination.

    • Conclusions:

    • The described automated procedure offers a significant advancement in DNA restriction fragment analysis.
    • The system provides high throughput, efficiency, and versatility for various molecular biology applications.