A near perfect temperature adaptation of bilayer order in vertebrate brain membranes

The bilayer order of a brain synaptic membrane fraction from a number of fish, mammalian and avian species have been compared in relation to their respective body temperatures using steady-state and time-resolved fluorescence anisotropy techniques. Fluorescence anisotropy for both 1,6-diphenyl-1,3,5-hexatriene and trans-parinaric acid increased in the order: antarctic Notothenia, trout, perch, cichlid, rat and starling, this also being the order of increasing body temperature. This suggests that cold-adapted fish species possess more disordered brain membranes than warm-adapted fish species, and mammals and birds membranes were more ordered than fish membranes. Comparison of temperature profiles for both fluorescence probes showed that fish species display similar anisotropies, and by inference bilayer order, to mammals and birds when measured at their respective body temperatures. Time-resolved analysis showed that the interspecific differences in (P2) order parameter was consistently related to body temperature whilst the rotational diffusion coefficient was not. These results suggest that brain membrane order is highly conserved within the vertebrates despite large differences in thermal habits and phylogenetic position. Polar fish species have by far the lowest bilayer order indicating that invasion of extreme cold habitats involved an adaptive decrease in bilayer order and conversely adoption of a high body temperature by mammals involved an adaptive increase in bilayer order. The conservation of membrane static order for these species at their respective body temperatures indicates a regulatory control of this aspect of membrane hydrocarbon structure and the functional importance of this structure.