Haploid specific activations of protamine 1 and hsc70t genes in mouse spermatogenesis

Protamine 1 and heat shock cognate 70 kDa protein (hsc70t) are known to be synthesized in haploid cells during spermatogenesis, and the mRNAs of these proteins have also been shown to accumulate in the haploid cells. However, it is unknown at which stage of spermatogenesis the genes for these proteins are actually activated. To examine this problem, we fractionated mouse adult testes cells at four different developmental stages, extracted their nuclei and carried out run-off assays with hsc70t and protamine 1 DNA probes. Results showed that both genes are mainly activated at the round spermatid stage. As the protein products of these genes accumulate at the later stage, it is interesting that these genes are regulated at the transcriptional and translational levels during spermatogenesis.