Uncorrected SCE levels of Bloom syndrome cells by cell hybridization with malignant cells with 14q32 structural...

Because 14q markers were frequently observed in lymphoid malignancies, we have done cell hybridization between Bloom syndrome (BS) cell lines and various malignant cell lines with and without 14q abnormalities, and noticed that BS cell hybrids which had been fused with cell lines involving 14q32 abnormalities did not decrease high SCE values in BS cells, as was observed in those of hybrids between homozygote BS. A constant fragile site was observed at 14q23 in bromodeoxyuridine (BrdU)-labeled hetero and homo BS cells. We considered the possible linkage of BrdU-induced fragility and SCE in BS cells.