Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prostaglandin E1 analogues misoprostol and enisoprost decrease microbial translocation and modulate the immune

L Gianotti1, J W Alexander, T Pyles

  • 1Department of Surgery, School of Medicine, University of Cincinnati, Ohio.

Circulatory Shock
|August 1, 1993
PubMed
Summary

High doses of misoprostol and enisoprost reduce bacterial translocation after burn injury in mice. Low doses, however, enhance bacterial clearance, indicating a dose-dependent effect of these prostaglandin E1 (PGE1) analogues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Calcineurin deficiency decreases inflammatory lesions in transforming growth factor beta1-deficient mice.

Clinical and experimental immunology·2009
Same author

Loss of the retinoblastoma tumor suppressor: differential action on transcriptional programs related to cell cycle control and immune function.

Oncogene·2007
Same author

Inhibition of melanosome transfer from melanocytes to keratinocytes by lectins and neoglycoproteins in an in vitro model system.

Pigment cell research·2001
Same author

Oral arginine improves blood pressure in renal transplant and hemodialysis patients.

JPEN. Journal of parenteral and enteral nutrition·2001
Same author

Nutritional immunomodulation leads to enhanced allograft survival in combination with cyclosporine A and rapamycin, but not FK506.

Transplantation·2000
Same author

Heat shock response: presence and effects in burn patient neutrophils.

Journal of leukocyte biology·1999

Area of Science:

  • Biomedical Science
  • Pharmacology
  • Immunology

Background:

  • Burn injuries compromise the integrity of the intestinal barrier, increasing the risk of bacterial translocation.
  • Bacterial translocation can lead to systemic infection and sepsis, exacerbating burn-related morbidity and mortality.
  • Prostaglandin E1 (PGE1) analogues are known for their cytoprotective effects, but their impact on bacterial translocation post-burn is not fully understood.

Purpose of the Study:

  • To investigate the effects of two PGE1 analogues, misoprostol and enisoprost, on bacterial translocation following a burn injury.
  • To determine if these PGE1 analogues modulate bacterial clearance in a dose-dependent manner.

Main Methods:

  • Balb/c mice were administered varying doses of misoprostol or enisoprost for three days before inducing a 20% full-thickness burn.

Related Experiment Videos

  • Mice were gavaged with 14C-labeled Escherichia coli to track bacterial translocation.
  • Tissues (blood, peritoneal fluid, lymph nodes, spleen, liver, lungs) were collected at 4 and 24 hours post-burn for radionuclide and bacterial enumeration.

    • Main Results:

    • High doses (200 micrograms/kg/day) of both misoprostol and enisoprost significantly decreased the translocation of 14C-E. coli into systemic tissues.
    • Low doses (20 micrograms/kg/day) of both analogues enhanced the clearance of translocated bacteria.
    • These findings indicate a dose-dependent modulation of bacterial translocation and clearance by PGE1 analogues.

    Conclusions:

    • Both misoprostol and enisoprost demonstrate the ability to reduce bacterial translocation following burn injury in a preclinical model.
    • The efficacy of these PGE1 analogues in managing bacterial translocation is dependent on the administered dose.
    • Further research into PGE1 analogues could offer novel therapeutic strategies for preventing or treating post-burn infections.