Microaffinity chromatographic separation and characterization of lipoprotein fractions in rat and mongolian gerbil...

A rapid, routine microaffinity chromatographic procedure with spectrometric detection was evaluated to measure the concentration of total and free cholesterol, triglycerides, and phospholipids linked to the alpha- and beta-/pre-beta-lipoproteins in very small serum samples from small experimental animals and from humans. This procedure overcomes many of the limitations presented by methods involving ultracentrifugation, electrophoresis, or precipitation. Using this method, we report that in 35 adult male Mongolian gerbils (Meriones unguiculatus) fed a basal commercial diet with no cholesterol supplementation, the mean (+/- SD) concentration of total and free cholesterol linked to the alpha-lipoprotein fraction (in mmol/L) is 2.85 +/- 0.81 and 0.33 +/- 0.14, respectively; phospholipids were 1.99 +/- 0.41, and triglycerides were 0.64 +/- 0.42. The concentration (mmol/L) of total and free cholesterol associated with the beta-/pre-beta-lipoprotein fraction is 0.92 +/- 0.26 and 0.22 +/- 0.06, respectively; phospholipids were 0.28 +/- 0.17, and triglycerides were 0.49 +/- 0.31.