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A reference preparation of creatine kinase BB isoenzyme
M Mathieu1, J P Steghens, M Hørder
1Hospices Civils de Lyon, Hôpital Debrousse, Service de Biochimie, France.
Clinical Chemistry
|September 1, 1993
Summary
A new reference material for creatine kinase isoenzyme BB (CK-BB) has been developed. This certified preparation aids in standardizing total creatine kinase (CK) assays for improved diagnostic accuracy in clinical chemistry.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Enzymology
Background:
- Serum creatine kinase (CK) catalytic activity is crucial for diagnosing conditions affecting the heart, muscle, and brain.
- Routine methods measure total CK, but organ-specific isoenzymes (CK-MM, CK-MB, CK-BB) vary.
- Standardized methods and reference materials are needed to ensure reliable CK measurements.
Purpose of the Study:
- To present a certified reference preparation of purified creatine kinase isoenzyme BB (CK-BB).
- To enable calibration of assays for total CK and its isoenzymes (CK-MM, CK-MB, CK-BB).
Main Methods:
- Purification of CK-BB isoenzyme from human placentae.
- Characterization of the lyophilized CK-BB preparation.
- Certification of the reference material (CRM 299) by the Community Bureau of Reference.
Main Results:
- A stable, lyophilized reference preparation of purified CK-BB was successfully developed.
- The preparation has been certified as CRM 299.
- This material can be used to calibrate various CK assay methods.
Conclusions:
- The certified CK-BB reference preparation enhances the standardization of creatine kinase measurements.
- Improved assay calibration leads to greater confidence in clinical chemistry results.
- This contributes to more accurate diagnosis and follow-up of various pathological conditions.