Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular engineering: applications to the clinical laboratory

R G Hamilton1

  • 1Clinical Immunology Division, Johns Hopkins Asthma and Allergy Center, Johns Hopkins University School of Medicine, Baltimore, MD 21224.

Clinical Chemistry
|September 1, 1993
PubMed
Summary

Engineered chimeric antibodies serve as versatile tools in clinical immunology labs. They are used for assay documentation, calibration, and studying autoantibody interference, improving diagnostic accuracy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cashew allergy, 2S albumins, and risk predictions based on IgE antibody levels.

Allergy·2016
Same author

EAACI Molecular Allergology User's Guide.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2016
Same author

Prevalence and risk factors for allergic rhinitis in two resource-limited settings in Peru with disparate degrees of urbanization.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2014
Same author

Resilience following spinal cord injury: a phenomenological view.

Spinal cord·2014
Same author

Provocation tests with objective measures remain more diagnostic than surrogate immunoglobulin E antibody measures of sensitization.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2011
Same author

Cat allergen-induced blood basophil reactivity in vitro predicts acute human nasal allergen challenge responses in vivo.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2011

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • Advancements in molecular biology enable the engineering of novel human biomolecules.
  • These engineered biomolecules have applications in documenting clinical laboratory assays.

Purpose of the Study:

  • To describe the use of engineered chimeric antibodies in clinical immunology.
  • To illustrate their applications as reference proteins, calibration proteins, and interference proteins.

Main Methods:

  • Utilized engineered chimeric antibodies with defined V-region specificity and various human C-region isotypes.
  • Developed mutant human IgG1-4 anti-dansyl chimeric antibodies for epitope mapping.
  • Employed a recombinant human IgG1 anti-human IgE Fc chimeric antibody to simulate autoantibody interference.

Related Experiment Videos

Main Results:

  • Demonstrated the utility of chimeric antibodies in documenting assay specificity and serving as reagent-grade antigens.
  • Showcased their role in calibrating antibody standards and mapping antibody epitopes.
  • Investigated the impact of autoantibodies on clinical assay accuracy and sensitivity using these engineered proteins.

Conclusions:

  • Engineered chimeric antibodies are valuable tools for clinical immunology laboratories.
  • They enhance assay validation, standardization, and the understanding of diagnostic interferences.