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Related Experiment Videos

Estrogen-responsive elements contain non-B DNA

D A Lannigan1, N J Koszewski, A C Notides

  • 1Department of Zoology, University of Vermont, Burlington 05405.

Molecular and Cellular Endocrinology
|July 1, 1993
PubMed
Summary

Estrogen-responsive elements (EREs) exhibit a non-B DNA conformation, indicating structural lability. This intrinsic property may facilitate estrogen receptor binding in vivo.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The estrogen receptor (ER) is a transcription factor regulating gene expression.
  • ER interacts with specific DNA sequences known as estrogen-responsive elements (EREs).

Purpose of the Study:

  • To investigate the DNA conformation of EREs.
  • To determine if EREs possess unique structural properties influencing estrogen receptor binding.

Main Methods:

  • Utilized chemical probing (potassium permanganate, osmium tetroxide, diethylpyrocarbonate) and S1 nuclease sensitivity assays.
  • Compared EREs with thyroid hormone-responsive elements to assess specificity.

Main Results:

  • EREs, both perfect and imperfect, adopt a non-B DNA conformation.
  • This non-B DNA structure is an intrinsic property of EREs, regardless of DNA topology.
  • S1 nuclease sensitivity was specific to EREs, not observed in other hormone-responsive elements.

Conclusions:

  • Nucleotides within EREs are structurally labile.
  • This inherent lability may form a unique DNA tertiary structure in vivo, aiding estrogen receptor recognition.

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