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Updated: Aug 10, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
Published on: May 12, 2015
Fluorescence microscopical visualization of rat mast cells by means of fluoresceine isothiocyanate labeled protamine
Abstract:
Using fluoresceine isothiocyanate labeled protamine sulfate it is possible to stain rat mast cell fractions of isolated periotoneal cells in a selective way. The cells are not degranulated, though they have lost their histamine completely. The method shows the dissociation of the mediator release and the extrusion of the granules.
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