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Purification of the pregnancy zone protein by affinity chromatography
Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry
|January 1, 1978
Abstract:
A method for purification of the pregnancy zone protein (PZP) by affinity chromatography was developed. A monospecific immunoglobulin fraction, covalently coupled to Sepharose 4B, was used as binding agent and the elution conditions for PZP are described. The purified protein was shown to have identical properties compared to native PZP with regard to molecular weight, immunodiffusion precipitation and immunosuppressive activity.