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Efficient plasmid mobilization by pIP501 in Lactococcus lactis subsp. lactis

P Langella1, Y Le Loir, S D Ehrlich

  • 1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

Journal of Bacteriology
|September 1, 1993
PubMed
Summary

Researchers identified a minimal mobilization (mob) locus on the pIP501 streptococcal conjugative plasmid. This locus facilitates high-frequency DNA transfer between gram-positive bacteria, aiding in understanding plasmid mobility.

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Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Streptococcal conjugative plasmid pIP501 exhibits broad host range transmission among gram-positive bacteria.
  • Understanding the genetic elements governing plasmid mobilization is crucial for bacterial genetics and biotechnology.

Purpose of the Study:

  • To identify and characterize the minimal mobilization (mob) locus responsible for pIP501 plasmid transfer.
  • To investigate the DNA sequences and genetic features within the mob locus.

Main Methods:

  • Cloning of pIP501 DNA fragments into nonconjugative plasmids.
  • Mobilization assays in Lactococcus lactis subsp. lactis strains.
  • DNA sequencing of the identified mobilization locus.

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Main Results:

  • A specific 1.0-kb DNA fragment containing the minimal mob locus was identified, conferring high-frequency mobilization.
  • The mob locus includes three palindromic sequences (palI, palII, palIII) and an open reading frame (ORFX).
  • The palI sequence shows homology with sequences in other conjugative plasmids like pAMbeta1 and is crucial for mobilization.

Conclusions:

  • The identified mob locus is essential for efficient conjugative transfer of pIP501.
  • The mob locus, particularly palI, plays a significant role in mediating plasmid mobilization and cointegrate formation.
  • These findings contribute to understanding the mechanisms of plasmid dissemination in gram-positive bacteria.