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Sensitive method for determination of nicorandil in human plasma by reversed-phase high-performance liquid
1Assay Technology Laboratory, Development and Technology Division, Chugai Phamaceutical Co. Ltd., Tokyo, Japan.
Journal of Chromatography
|July 23, 1993
Abstract:
A sensitive method for the determination of nicorandil, an anti-anginal drug, has been developed. The method involves solid-phase extraction of the drug and an internal standard using a Bond-Elut PH extraction column, liquid-liquid extraction with methyl acetate and dichloromethane, and reversed-phase high-performance liquid chromatography on a mu Bondapak C18 column with an ultraviolet detector. The limit of determination in plasma was 3 ng/ml and the intra-day coefficient of variation (n = 7) was less than 10%.