Complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus

The complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (ACLSVA) was determined. The genome is 7552 nucleotides excluding the poly(A) tail and contains three open reading frames (ORFs 1, 2 and 3), encoding proteins with M(r) values of 216,503 (216.5K), 50,453 (50.4K) and 21,394 (21.4K), respectively. Nucleotide sequence comparisons between ACLSV-A and the previously sequenced ACLSV from plum (ACLSV-P) showed that the sequence identity at the nucleotide level was 79.8%. Amino acid sequence identities of ORFs 1 and 2 between both isolates were 88.4% and 79.9%, respectively. The 21.4K protein encoded by ORF 3 of ACLSV-A had an amino acid sequence identity of 88.6% with the 28.3K protein encoded by ORF 3 of ACLSV-P. Immunoblot analysis of the 21.4K protein expressed in Escherichia coli showed that this protein is the coat protein of ACLSV-A.