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Related Experiment Videos

Calbindin-D28k gene expression in the developing mouse kidney

L Liu1, S T Dunn, S Christakos

  • 1Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.

Kidney International
|August 1, 1993
PubMed
Summary
This summary is machine-generated.

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Calbindin-D28k mRNA is found in developing mouse kidneys, specifically in ureteric bud cells during early renal morphogenesis. Its distribution mirrors the protein

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Nephrology

Background:

  • Calbindin-D28k is a calcium-binding protein present in the metanephric kidney during embryogenesis.
  • Understanding its expression pattern is crucial for comprehending kidney development.

Purpose of the Study:

  • To investigate the temporal and spatial expression of calbindin-D28k mRNA during mouse kidney development.
  • To correlate mRNA distribution with protein localization and its role in renal morphogenesis.

Main Methods:

  • In situ hybridization using a 35S-UTP-labeled antisense cRNA probe for calbindin-D28k mRNA.
  • Analysis of developing mouse kidneys from embryonic day 12 to postnatal day 21.

Main Results:

Related Experiment Videos

  • Calbindin-D28k mRNA was detected in ureteric buds starting at embryonic day 12, with intense labeling in advancing buds and ampullae.
  • Labeling was observed in distal nephron segments but not in renal vesicles, proximal tubules, or Henle's loop.
  • By postnatal day 21, collecting ducts and ureter were no longer labeled.

    • Conclusions:

    • Calbindin-D28k mRNA expression in the developing kidney is localized to the ureteric bud lineage.
    • The spatial distribution of calbindin-D28k mRNA parallels that of the protein during nephrogenesis.
    • The calbindin-D28k gene is transcribed in ureteric bud cells during early renal morphogenesis.